Johanne E Spreckels

Mar 1, 2024 | nature.com | Sanzhima Garmaeva |Trishla Sinha |Anastasia A. Gulyaeva |Johanne E Spreckels |Sergio Andreu-Sánchez |Ranko Gacesa | +8 more

AbstractEarly development of the gut ecosystem is crucial for lifelong health. While infant gut bacterial communities have been studied extensively, the infant gut virome remains under-explored. To study the development of the infant gut virome over time and the factors that shape it, we longitudinally assess the composition of gut viruses and their bacterial hosts in 30 women during and after pregnancy and in their 32 infants during their first year of life.

Nov 8, 2023 | nature.com | Johanne E Spreckels |Hermie Harmsen |Jingyuan Fu

AbstractHuman milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We tested four DNA isolation methods, two bacterial enrichment methods and three sequencing methods on mock communities, milk samples and negative controls.