Hongmiao Hu

Aug 25, 2023 | science.org | Yun Zhu |Hideaki Yamamoto |David Elser |Hongmiao Hu

MATERIALS AND METHODSHuman histone H2A/H2B dimer was purified as described previously (43). Plasmid pRSFDuet-H3-H4 (a gift from K. Muir and D. Barford, Medical Research Council, Laboratory of Molecular Biology) with an N-terminal His6-HRV-3C site fusion on H4 was transformed into Rosetta(DE3)-pLysS cells. HRV-3C is human rhinovirus 3C protease cleavage site.