Janine Kamps

Apr 22, 2024 | jbc.org | Janine Kamps

AbstractLiquid-liquid phase separation (LLPS) of the mammalian prion protein is mainly driven by its intrinsically disordered N-terminal domain (N-PrP). However, the specific intermolecular interactions that promote LLPS remain largely unknown. Here, we used extensive mutagenesis and comparative analyses of evolutionarily distant PrP species to gain insight into the relationship between protein sequence and phase behavior.

Jul 12, 2023 | pubs.rsc.org | Sashary Ramos |Janine Kamps |Simone Pezzotti |Konstanze F. Winklhofer

Hydration makes a difference! How to tune protein complexes between liquid-liquid and liquid-solid phase separation. Understanding the mechanisms that dictate Liquid-Liquid Phase Separation (LLPS) and Liquid-Solid Phase Separation (LSPS) and the transition between the two are prominent challenges in biology and medicine.