Jared O. Kafader

Jan 2, 2025 | nature.com | Jared O. Kafader

AbstractIndividual ion mass spectrometry (I2MS) is the Orbitrap-based extension of the niche mass spectrometry technique known as charge detection mass spectrometry (CDMS). While traditional CDMS analysis is performed on in-house-built instruments such as the electrostatic linear ion trap, I2MS extends CDMS analysis to Orbitrap analyzers, allowing charge detection analysis to be available to the scientific community at large.

Dec 1, 2023 | nature.com | John McGee |Pei Su |Nicholas W Bateman |Thomas P Conrads |Jeannie M. Camarillo |Jared O. Kafader

Correction to: Nature Communications https://doi.org/10.1038/s41467-023-42208-3, published online 14 October 2023The original version of the Article contained the following errors in Fig. 2: the proteoform signature ‘7125 Da’ was incorrectly labelled as ‘PSMD1’ in panels b and c; the labels ‘237 tumor pixels’ and ‘235 stroma pixels’ were incorrectly added to panel d and they have been removed in the correct version. The correct version of Fig.

Oct 14, 2023 | nature.com | John McGee |Pei Su |Nicholas W Bateman |Thomas P Conrads |Jeannie M. Camarillo |Jared O. Kafader

AbstractThe molecular identification of tissue proteoforms by top-down mass spectrometry (TDMS) is significantly limited by throughput and dynamic range. We introduce AutoPiMS, a single-ion MS based multiplexed workflow for top-down tandem MS (MS2) directly from tissue microenvironments in a semi-automated manner. AutoPiMS directly off human ovarian cancer sections allowed for MS2 identification of 73 proteoforms up to 54 kDa at a rate of <1 min per proteoform.