Jean Fan

3 weeks ago | biorxiv.org | Caleb Hallinan |Hyun Ji |Steven Salzberg |Jean Fan

AbstractThe accuracy of spatial gene expression profiles generated by probe-based in situ spatially-resolved transcriptomic technologies depends on the specificity with which probes bind to their intended target gene. Off-target binding, defined as a probe binding to something other than the target gene, can distort a gene's true expression profile, making probe specificity essential for reliable transcriptomics.