Jiahong Wang

Jun 23, 2023 | genomebiology.biomedcentral.com | Xin Zhang |Xinlong Wang |Jie Lv |Hongxin Huang |Jiahong Wang |Zhihong Tan | +9 more

Plasmid constructionFor U6+27 gRNA-expressing constructs, the DNA sequences including the hU6+27 promoter, a gRNA scaffold, and a gRNA insert site were synthesized and cloned into the pBluSKM vector. For Pre gRNA-expressing constructs, a second gRNA scaffold was inserted downstream of the gRNA insert site in the U6+27 gRNA-expressing constructs.