Jie Lv

Jun 23, 2023 | genomebiology.biomedcentral.com | Xin Zhang |Xinlong Wang |Jie Lv |Hongxin Huang |Jiahong Wang |Zhihong Tan | +9 more

Plasmid constructionFor U6+27 gRNA-expressing constructs, the DNA sequences including the hU6+27 promoter, a gRNA scaffold, and a gRNA insert site were synthesized and cloned into the pBluSKM vector. For Pre gRNA-expressing constructs, a second gRNA scaffold was inserted downstream of the gRNA insert site in the U6+27 gRNA-expressing constructs.

May 11, 2023 | dx.doi.org | Kai Chen |Jie Lv |Jie Chen |Jian Zhang

An efficient Au(III)/Ag(I) co-catalytic platform has been established for the synthesis of cyclopentadienes through amine-release annulation of enaminones with alkynes. Vinylcarbenoids that were generated from 1,2-migration of propargyl esters may undergo a tandem annulation process with enaminones to give aminocyclopentenes as key intermediates. The bimetal catalytic system is compatible with a broad substrate scope under mild reaction conditions.