Jonathan L. Schmid-Burgk

Dec 19, 2024 | nature.com | Jonathan L. Schmid-Burgk

AbstractOptical pooled screening offers a broader-scale alternative to enrichment-based perturbation screening, using fluorescence microscopy to correlate phenotypes and perturbations across single cells. Previous methods work well in large, transcriptionally active cell lines, because they rely on cytosolic detection of endogenously expressed barcoded transcripts; however, they are limited by reliable cell segmentation, cytosol size, transcriptional activity and cell density.

Sep 7, 2023 | nature.com | Zuzanna Kozicka |Katherine A. Donovan |Marko Cigler |Jonathan L. Schmid-Burgk |Daniel Häussinger |Georg E. Winter | +4 more

AbstractMolecular glue degraders are an effective therapeutic modality, but their design principles are not well understood. Recently, several unexpectedly diverse compounds were reported to deplete cyclin K by linking CDK12–cyclin K to the DDB1–CUL4–RBX1 E3 ligase.