Joshua A. Bull

Dec 8, 2024 | biorxiv.org | Helen Byrne |Joshua A. Bull |Joshua Moore |Eoghan J. Mulholland

AbstractThe generation of spatial data in biology has been transformed by multiplex imaging and spatial-omics technologies, such as single cell spatial transcriptomics. These approaches permit detailed mapping of phenotypic information about individual cells and their spatial locations within tissue sections.

Jun 3, 2024 | biorxiv.org | Helen Byrne |Joshua A. Bull |Eoghan J. Mulholland |Joshua Moore

AbstractSpatial biology has the potential to unlock information about the disrupted cellular ecosystems that define human disease. Quantitative analysis of spatially-resolved cell interactions allows mapping of tissue self-organisation and assessment of why cells interact differently in physiological and pathological contexts.

Apr 24, 2024 | nature.com | Mike B. Barnkob |Yale Michaels |Philip Macklin |Uzi Gileadi |Salvatore Valvo |Margarida Rei | +14 more

Correction to: Nature Communications https://doi.org/10.1038/s41467-024-47424-z, published online 12 April 2024In this article the title was incorrectly written as ‘Semmaphorin 3A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells’ but should have been ‘Semaphorin 3A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells’. The original article has been corrected.

Apr 12, 2024 | nature.com | Yale Michaels |Philip Macklin |Uzi Gileadi |Salvatore Valvo |Margarida Rei |Corinna A. Kulicke | +14 more

AbstractSemaphorin-3A (SEMA3A) functions as a chemorepulsive signal during development and can affect T cells by altering their filamentous actin (F-actin) cytoskeleton. The exact extent of these effects on tumour-specific T cells are not completely understood. Here we demonstrate that Neuropilin-1 (NRP1) and Plexin-A1 and Plexin-A4 are upregulated on stimulated CD8+ T cells, allowing tumour-derived SEMA3A to inhibit T cell migration and assembly of the immunological synapse.

Nov 8, 2023 | nature.com | Joshua A. Bull |Martin Sergeant |Rachel Etherington |Fadi Issa |Ignacio Melero Bermejo |Simon J. McGowan | +11 more

AbstractSingle cell spatial interrogation of the immune-structural interactions in COVID −19 lungs is challenging, mainly because of the marked cellular infiltrate and architecturally distorted microstructure. To address this, we develop a suite of mathematical tools to search for statistically significant co-locations amongst immune and structural cells identified using 37-plex imaging mass cytometry.