Kade Pettie

Jan 15, 2024 | genomebiology.biomedcentral.com | Kade Pettie |Maxwell R. Mumbach |Amanda Lea |Julien Ayroles |Howard Chang |Hunter Fraser | +1 more

For detailed methods on cell culture conditions and processing, see our previous study [19]. Briefly, 2×103 cells from each LCL were collected and pooled by population after growth to 6–8×105 cells/mL. To prevent disproportionate cell line growth within pools throughout the collection and pooling process, sub-pools were frozen in liquid nitrogen at −180 °C.