Kaiyi Li

Jun 19, 2024 | onlinelibrary.wiley.com | Chang Liu |Kaiyi Li |Xizhao Sui |Tian Zhao

Construction and Optimization of the Automated Single-Cell Distribution Instrument The home-built single-cell distribution instrument (SCDI) consisting of an integrated microscopy module for imaging cells, a six-axis motion and control module for operating the chip platform and the robotic arm, and a motor-controlled hydraulic microneedle module for cell delivery.

Jun 6, 2024 | biorxiv.org | Kaiyi Li |Chang Liu |Ting Zhang |Tian Zhao

AbstractDeciphering the interactions between tumor micro- and systemic immune macro-environment is essential for developing more effective cancer diagnosis and treatment strategies. Here, we established a gel-liquid interface (GLI) co-culture of lung cancer organoids (LCOs) and paired peripheral blood mononuclear cells (PBMCs), featuring with enhanced interactions of immune cells and tumor organoids, to mimic the in vivo systemic anti-tumor immunity induced by immune checkpoint inhibitors (ICI).

Dec 12, 2023 | biorxiv.org | Chang Liu |Kaiyi Li |Xizhao Sui |Tian Zhao

AbstractIn vitro models coupled with multimodal approaches are urgently needed to decipher the local tumor immune microenvironment (TIME) owing to the heterogeneous nature of immune cells and their diverse spatial distributions. Here we generate primary lung cancer organoids (pLCOs) by isolating the tumor cell clusters, including the infiltrated immune cells, from dissected lung cancer samples.