Kira Behrens

Feb 7, 2024 | nature.com | Joshua Hardy |Kira Behrens |Tamanna Saiyed |Leila Jebeli |Nichollas E Scott |Jeffrey J. Babon

AbstractThrombopoietin (Tpo) is the primary regulator of megakaryocyte and platelet numbers and is required for haematopoetic stem cell maintenance. Tpo functions by binding its receptor (TpoR, a homodimeric Class I cytokine receptor) and initiating cell proliferation or differentiation. Here we characterise the murine Tpo:TpoR signalling complex biochemically and structurally, using cryo-electron microscopy. Tpo uses opposing surfaces to recruit two copies of receptor, forming a 1:2 complex.