Kun Shi

Nov 29, 2024 | science.org | Young-Cheul Shin |Yang Wu |Chaojiao Sun |Kun Shi

AbstractEngineering nonribosomal peptide synthetases (NRPSs) has been a “holy grail” in synthetic biology due to their modular nature and limited understanding of catalytic mechanisms. Here, we reported a computational redesign of the “gate-keeper” adenylation domain of the model NRPS-like enzyme carboxylic acid reductases (CARs) by using approximate mechanism-based geometric criteria and the Rosetta energy score.

Apr 29, 2024 | mdpi.com | Yi Tan |Xiaoli Wang |Jason Lee |Kun Shi

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Oct 24, 2023 | onlinelibrary.wiley.com | Meng Wang |DanRong Hu |Yun Yang |Kun Shi

1 Introduction Colorectal cancer (CRC) is the most prevalent malignant tumor of the digestive system and is characterized by high morbidity and mortality rates.[1] The low early diagnosis rate of CRC can be attributed to ambiguous abdominal symptoms and a lack of endoscopic resources.[2] At the initial diagnosis, distant metastasis is present in 44% of patients.

Oct 16, 2023 | nature.com | Jian Zhou |Peter R. Leavitt |Kevin Rose |Kun Shi

AbstractAtmospheric warming heats lakes, but the causes of variation among basins are poorly understood. Here, multi-decadal profiles of water temperatures, trophic state, and local climate from 345 temperate lakes are combined with data on lake geomorphology and watershed characteristics to identify controls of the relative rates of temperature change in water (WT) and air (AT) during summer.