Lauryn Cook

Oct 31, 2024 | digitalcommons.library.tmc.edu | Alison Bates |Lauryn Cook |Rebecca B Stowe |Elizabeth M Travis

Publication Date6-4-2023JournalBiochemical and Biophysical Research CommunicationsAbstractKCNQ1, the major component of the slow-delayed rectifier potassium channel, is responsible for repolarization of cardiac action potential. Mutations in this channel can lead to a variety of diseases, most notably long QT syndrome. It is currently unknown how many of these mutations change channel function and structure on a molecular level.

Feb 20, 2024 | cell.com | Jian Yang |Lauryn Cook |Zhiyuan Chen

Highlights•Zygotic injection of dCas9-KRAB mRNA achieves robust CRISPRi in 2-cell embryos•MT2 CRISPRi efficiently perturbs thousands of MT2/MERVL copies during mouse ZGA•MT2 CRISPRi causes ZGA and preimplantation defects•MT2-derived promoters/enhancers function downstream of OBOX/DUX proteinsSummaryIn mammals, many retrotransposons are de-repressed during zygotic genome activation (ZGA).

Sep 20, 2023 | biorxiv.org | Jian Yang |Zhiyuan Chen |Lauryn Cook

AbstractIn mammals, many retrotransposons are de-repressed during zygotic genome activation (ZGA). However, their functions in early development remain elusive largely due to the challenge to simultaneously manipulate thousands of retrotransposon insertions in embryos. Here, we employed epigenome editing to perturb the long terminal repeat (LTR) MT2_Mm, a well-known ZGA and totipotency marker that exists in ~2667 insertions throughout the mouse genome.