Lishomwa C. Ndhlovu

Apr 9, 2024 | nature.com | Wen Hu |Mark Diekhans |Jordan Marrocco |Lishomwa C. Ndhlovu |Teresa A. Milner |Erich D. Jarvis | +3 more

AbstractRNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes, developmental time points and species. For 72% of genes, full-length isoform expression varies along one or more axes. Splicing, transcription start and polyadenylation sites vary strongly between cell types, influence protein architecture and associate with disease-linked variation.