Longjian Niu

Aug 18, 2024 | biorxiv.org | Ying Chen |Bo Wu |Yuying Ding |Longjian Niu

AbstractPacBio Circular Consensus Sequencing (CCS) allows us to obtain highly accurate bases and simultaneously determine the methylation states of individual molecules. However, existing CCS-based methods for 5mC detection have low accuracy (<90% on most datasets) at the single-molecule level and can produce inaccurate methylation patterns.