Lori Mandjikian

Feb 4, 2024 | biorxiv.org | Jonathan T. Vu |Katherine U Tavasoli |Lori Mandjikian |Connor J Sheedy

AbstractPeroxisomes are membrane-bound organelles harboring metabolic enzymes. In humans, peroxisomes are required for normal development, yet the genes regulating peroxisome function remain unclear. We performed a genome-wide CRISPRi screen to identify novel factors involved in peroxisomal homeostasis. We found that inhibition of RNF146, an E3 ligase activated by poly(ADP-ribose), reduced the import of proteins into peroxisomes.