Lucinda Barrett

Oct 25, 2024 | microbiologyresearch.org | Gillian Rodger |Samuel Lipworth |Lucinda Barrett |Sarah Oakley

Article metrics loading... /content/journal/mgen/10.1099/mgen.0.001296Download as PowerPoint /deliver/fulltext/mgen/10/10/mgen001296.html?itemId=/content/journal/mgen/10.1099/mgen.0.001296&mimeType=html&fmt=ahah References Bayega A, Oikonomopoulos S, Wang YC, Ragoussis J. Improved nanopore full-length cDNA sequencing by PCR-suppression. Front Genet 2022; 13:1031355 [View Article] [PubMed][Google Scholar] Stark R, Grzelak M, Hadfield J. RNA sequencing: the teenage years.

May 17, 2024 | medrxiv.org | Samuel Lipworth |Kevin Chau |Sarah Oakley |Lucinda Barrett

The authors have declared no competing interest. The computational aspects of this research were funded from the NIHR Oxford BRC with additional support from the Wellcome Trust Core Award Grant Number 203141/Z/16/Z. SL was funded by an MRC Clinical Research Training Fellowship MR/T001151/1. ASW and TEAP are also supported by the NIHR Oxford Biomedical Research Centre. ASW is an NIHR Senior Investigator. NS is an NIHR Oxford BRC Senior Fellow.

Jan 23, 2024 | biorxiv.org | Gillian Rodger |Samuel Lipworth |Lucinda Barrett |Sarah Oakley

AbstractWhole-transcriptome (long-read) RNA sequencing (Oxford Nanopore Technologies, ONT) holds promise for agnostic analysis of differential gene expression (DGE) in pathogenic bacteria, including for antimicrobial resistance genes (ARGs). However, direct cDNA ONT sequencing requires large concentrations of polyadenylated mRNA, and amplification protocols may introduce technical bias.