Gillian Rodger

Oct 25, 2024 | microbiologyresearch.org | Gillian Rodger |Samuel Lipworth |Lucinda Barrett |Sarah Oakley

Article metrics loading... /content/journal/mgen/10.1099/mgen.0.001296Download as PowerPoint /deliver/fulltext/mgen/10/10/mgen001296.html?itemId=/content/journal/mgen/10.1099/mgen.0.001296&mimeType=html&fmt=ahah References Bayega A, Oikonomopoulos S, Wang YC, Ragoussis J. Improved nanopore full-length cDNA sequencing by PCR-suppression. Front Genet 2022; 13:1031355 [View Article] [PubMed][Google Scholar] Stark R, Grzelak M, Hadfield J. RNA sequencing: the teenage years.

Aug 12, 2024 | biorxiv.org | William Matlock |Gillian Rodger |Emma Pritchard |Matthew Colpus

AbstractCo-amoxiclav resistance in E. coli is a clinically important phenotype associated with increased mortality. The class A beta-lactamase blaTEM-1 is often carried by co-amoxiclav-resistant pathogens, but exhibits high phenotypic heterogeneity, making genotype-phenotype predictions challenging. We present a curated dataset of n=377 E. coli isolates representing all 8 known phylogroups, where the only acquired beta-lactamase is blaTEM-1.

Jan 23, 2024 | biorxiv.org | Gillian Rodger |Samuel Lipworth |Lucinda Barrett |Sarah Oakley

AbstractWhole-transcriptome (long-read) RNA sequencing (Oxford Nanopore Technologies, ONT) holds promise for agnostic analysis of differential gene expression (DGE) in pathogenic bacteria, including for antimicrobial resistance genes (ARGs). However, direct cDNA ONT sequencing requires large concentrations of polyadenylated mRNA, and amplification protocols may introduce technical bias.