Luisa Statello

Jul 24, 2023 | nature.com | Aina Maria Mas |Enrique Goñi |Aida Arcas |Luisa Statello |Dipika Gupta |Jernej Ule | +3 more

Cell lines, growth conditions, and culture treatmentsHCT116 cells (CCL-247) were cultured in RPMI-1640 (GIBCO), and U2OS cells (HTB-96) in DMEM (GIBCO), all mediums supplemented with 10% fetal bovine serum (GIBCO) and 1x penicillin/streptomycin (Lonza), and maintained at 37 ˚C and 5% CO2.

Mar 7, 2023 | biorxiv.org | Aina Maria Mas |Enrique Goñi |Aida Arcas |Luisa Statello

AbstractCells must coordinate the activation of thousands of replication origins dispersed throughout their genome. Active transcription is known to favor the formation of mammalian origins, although the role that RNA plays in this process remains unclear. We show that the ORC1 subunit of the human Origin Recognition Complex interacts with RNAs transcribed from genes with origins in their transcription start sites (TSSs), displaying a positive correlation between RNA binding and origin activity.