Magdalena Zernicka-Goetz

Jul 19, 2024 | biorxiv.org | Estefania Sanchez-Vasquez |Magdalena Zernicka-Goetz |Marianne E. Bronner

AbstractHuman fertility is suboptimal, partly due to error-prone divisions in early cleavage-stages that result in aneuploidy. Most human pre-implantation are mosaics of euploid and aneuploid cells, however, mosaic embryos with a low proportion of aneuploid cells have a similar likelihood of developing to term as fully euploid embryos. How embryos manage aneuploidy during development is poorly understood.

Dec 5, 2023 | nature.com | Magdalena Zernicka-Goetz

The creation of multiple stem-cell-derived models of mammalian embryogenesis is opening many new doors to study human development and brings a need for scientists to demonstrate responsible dialog over the associated ethical issues. Fertilization of the egg initiates the dance of life.

Oct 11, 2023 | nature.com | Magdalena Zernicka-Goetz

AbstractThe interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.

Sep 5, 2023 | biorxiv.org | Estefania Sanchez-Vasquez |Magdalena Zernicka-Goetz |Marianne E. Bronner

AbstractWe previously modeled elimination of aneuploid cells in mouse embryos using the Mps1 inhibitor reversine. Here, we develop an alternative model using AZ3146, a highly specific Mps1 inhibitor, to understand survival of mosaic embryos. AZ3146 treated embryos overexpress Hypoxia-Inducible-Factor-1A(HIF1A), inhibition of which results in a decreased cell number of extra-embryonic trophectoderm but not embryonic epiblast.

Aug 8, 2023 | nature.com | Joachim De Jonghe |Tomasz S Kaminski |Anna L. Ellermann |Timo N. Kohler |Gregory M. Findlay |Magdalena Zernicka-Goetz | +1 more

AbstractDroplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA capture efficiency is lower.