Maico Lechner

Oct 18, 2024 | biorxiv.org | Maico Lechner |Tanveer Singh Batth |Jesper V. Olsen |Cristina Hernandez-Rollan

AbstractThis study presents a comparative analysis of three LysC homologues from Achromobacter lyticus, Pseudomonas aeruginosa, and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture (PAC) workflow with HeLa cell lysate, we assessed the enzymes' cleavage specificity, digestion efficiency, and performance across various experimental conditions.