Manuela K. Hospenthal

Apr 8, 2024 | nature.com | Dawid Zyla |Paul Bachmann |Rafal Zdanowicz |Christoph Giese |Manuela K. Hospenthal

AbstractType 1 pili are important virulence factors of uropathogenic Escherichia coli that mediate bacterial attachment to epithelial cells in the urinary tract. The pilus rod is comprised of thousands of copies of the main structural subunit FimA and is assembled in vivo by the assembly platform FimD. Although type 1 pilus rods can self-assemble from FimA in vitro, this reaction is slower and produces structures with lower kinetic stability against denaturants compared to in vivo-assembled rods.