Mareike S. Stephan

Aug 19, 2024 | biorxiv.org | Mareike S. Stephan |Nina K. Broeker |Tom Robinson |Rumiana Dimova

AbstractGiant unilamellar vesicles (GUVs) are a versatile platform to study cell membrane functions. We have constructed phospholipid GUVs presenting lipopolysaccharide on their external surface (LPS-GUVs) to mimic the outer membrane (OM) of Gram-negative bacteria. GUVs allow for adjusting a defined OM composition, unlike the dynamic changes of LPS structures typically observed in vivo. The OM is a major control point for the viral genome transfer from bacteriophages into bacterial hosts.