Megan Lee

Oct 18, 2023 | biorxiv.org | Neal Bennett |Megan Lee |Adam Orr |Ken Nakamura

AbstractRespiratory chain dysfunction can decrease ATP and increase reactive oxygen species (ROS) levels. Despite the importance of these metabolic parameters to a wide range of cellular functions and disease, we lack an integrated understanding of how they are differentially regulated. To address this question, we adapted a CRISPRi- and FACS- based platform to compare the effects of respiratory gene knockdown on ROS to their effects on ATP.