Michael T. Eadon

May 8, 2024 | nature.com | Sanjay Jain |Michael T. Eadon

AbstractThe ability to localize hundreds of macromolecules to discrete locations, structures and cell types in a tissue is a powerful approach to understand the cellular and spatial organization of an organ. Spatially resolved transcriptomic technologies enable mapping of transcripts at single-cell or near single-cell resolution in a multiplex manner. The rapid development of spatial transcriptomic technologies has accelerated the pace of discovery in several fields, including nephrology.

Jul 19, 2023 | nature.com | Seth Winfree |Qiwen Hu |Ricardo Melo Ferreira |Daria Barwinska |fei chen |Sushrut S. Waikar | +12 more

Statistics and reproducibilityFor 3D imaging and immunofluorescence staining experiments, each staining was repeated on at least two separate individuals or separate regions. For immunofluorescence validation studies, commercially available antibodies were used; 13 out of the 15 tissue samples were also analysed using snCv3 or scCv3. For ISH, 6 tissue samples (4 biopsies and 2 nephrectomies) were analysed.