Milena Parlak

Oct 13, 2023 | biorxiv.org | Matthew Day |Bilal Tetik |Milena Parlak |Yasser Almeida-Hernandez

AbstractActivation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for replication origin firing, and as such for faithful genetic inheritance. Our biochemical and structural studies demonstrate that the helicase activator GINS interacts with TopBP1 through two separate binding surfaces, the first involving a stretch of highly conserved amino acids in the TopBP1-GINI region, the second a surface on TopBP1-BRCT4.