Yasser Almeida-Hernandez

Feb 27, 2024 | nature.com | Matthew Day |Bilal Tetik |Yasser Almeida-Hernandez |Markus Räschle |Farnusch Kaschani |Markus Kaiser | +2 more

AbstractActivation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for replication origin firing, and as such for faithful genetic inheritance. Our biochemical and structural studies demonstrate that the helicase activator GINS interacts with TopBP1 through two separate binding surfaces, the first involving a stretch of highly conserved amino acids in the TopBP1-GINI region, the second a surface on TopBP1-BRCT4.

Feb 2, 2024 | biorxiv.org | Joel Brenner |Yasser Almeida-Hernandez |Farnusch Kaschani |Linda Charlotte Zirden

AbstractHsp70/Hsp90-chaperones and their regulatory co-chaperones are critical for maintaining protein homeostasis. GRP94, the sole Hsp90-chaperone in the secretory pathway of mammalian cells, is essential for the maturation of important secretory and transmembrane proteins. Without the requirement of co-chaperones, the Hsp70-protein BiP controls regulatory conformational changes of GRP94 – the structural basis of which has remained elusive.

Oct 13, 2023 | biorxiv.org | Matthew Day |Bilal Tetik |Milena Parlak |Yasser Almeida-Hernandez

AbstractActivation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for replication origin firing, and as such for faithful genetic inheritance. Our biochemical and structural studies demonstrate that the helicase activator GINS interacts with TopBP1 through two separate binding surfaces, the first involving a stretch of highly conserved amino acids in the TopBP1-GINI region, the second a surface on TopBP1-BRCT4.