Mingyi Chen

Nov 6, 2024 | digitalcommons.library.tmc.edu | Yuxuan Liu |Lihu Gong |Mingyi Chen |Junhua Lyu

Mutations in IDH genes occur frequently in acute myeloid leukemia (AML) and other human cancers to generate the oncometabolite R-2HG. Allosteric inhibition of mutant IDH suppresses R-2HG production in a subset of patients with AML; however, acquired resistance emerges as a new challenge, and the underlying mechanisms remain incompletely understood. Here we establish isogenic leukemia cells containing common IDH oncogenic mutations by CRISPR base editing.