Mira Mastoras

2 months ago | digitalcommons.library.tmc.edu | Mikhail Kolmogorov |Kimberley Billingsley |Mira Mastoras |Melissa Meredith

KeywordsHumans, Sequence Analysis, DNA, Haplotypes, Genome, Human, Nanopore Sequencing, Methylation, Pilot Projects, High-Throughput Nucleotide SequencingAbstractLong-read sequencing technologies substantially overcome the limitations of short-reads but have not been considered as a feasible replacement for population-scale projects, being a combination of too expensive, not scalable enough or too error-prone.

Sep 14, 2023 | nature.com | Mikhail Kolmogorov |Kimberley Billingsley |Mira Mastoras |Melissa Meredith |Ramita Dewan |Kensuke Daida | +13 more

AbstractLong-read sequencing technologies substantially overcome the limitations of short-reads but have not been considered as a feasible replacement for population-scale projects, being a combination of too expensive, not scalable enough or too error-prone. Here we develop an efficient and scalable wet lab and computational protocol, Napu, for Oxford Nanopore Technologies long-read sequencing that seeks to address those limitations.