Naiya R Phillips

Apr 11, 2024 | biorxiv.org | Noam Prywes |Naiya R Phillips |Luke M Oltrogge |Sebastian Lindner

AbstractRubisco is the primary CO2 fixing enzyme of the biosphere yet has slow kinetics. The roles of evolution and chemical mechanism in constraining the sequence landscape of rubisco remain debated. In order to map sequence to function, we developed a massively parallel assay for rubisco using an engineered E. coli where enzyme function is coupled to growth.

Sep 27, 2023 | biorxiv.org | Noam Prywes |Luke M Oltrogge |Benoit de Pins |Naiya R Phillips

AbstractThe enzyme rubisco catalyzes the first step of carbon assimilation in photosynthesis. Despite the massive flux of CO2 passing through this active site over billions of years, extant rubisco has relatively slow kinetics and is prone to off-target activity. In many growth regimes, this limits photosynthesis in planta. Many attempts have been made to improve the kinetic parameters of rubisco with limited success, potentially due to biochemical trade-offs.