Noam Prywes

Apr 11, 2024 | biorxiv.org | Noam Prywes |Naiya R Phillips |Luke M Oltrogge |Sebastian Lindner

AbstractRubisco is the primary CO2 fixing enzyme of the biosphere yet has slow kinetics. The roles of evolution and chemical mechanism in constraining the sequence landscape of rubisco remain debated. In order to map sequence to function, we developed a massively parallel assay for rubisco using an engineered E. coli where enzyme function is coupled to growth.

Feb 21, 2024 | nature.com | David Ding |Nathan Rollins |Noam Prywes |David Savage |Michael T. Laub

AbstractRecent developments in protein design rely on large neural networks with up to 100s of millions of parameters, yet it is unclear which residue dependencies are critical for determining protein function. Here, we show that amino acid preferences at individual residues—without accounting for mutation interactions—explain much and sometimes virtually all of the combinatorial mutation effects across 8 datasets (R2 ~ 78-98%).

Dec 14, 2023 | biorxiv.org | Chintan Soni |Noam Prywes |Matthew Hall |David Savage

AbstractUsing directed evolution, engineered aminoacyl-tRNA synthetases (aaRS) have been developed that enable co-translational incorporation of numerous noncanonical amino acids (ncAAs) into proteins in living cells. Until now, the selection of such novel aaRS mutants has relied on coupling their activity to the expression of a reporter protein with a selectable phenotype.

Sep 27, 2023 | biorxiv.org | Noam Prywes |Luke M Oltrogge |Benoit de Pins |Naiya R Phillips

AbstractThe enzyme rubisco catalyzes the first step of carbon assimilation in photosynthesis. Despite the massive flux of CO2 passing through this active site over billions of years, extant rubisco has relatively slow kinetics and is prone to off-target activity. In many growth regimes, this limits photosynthesis in planta. Many attempts have been made to improve the kinetic parameters of rubisco with limited success, potentially due to biochemical trade-offs.

Jun 14, 2023 | biorxiv.org | David Ding |Ada Shaw |Nathan Rollins |Noam Prywes

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