Neti Bhatt

Nov 20, 2024 | biorxiv.org | Robert Miller |Michael Patterson |Neti Bhatt |Xiaokun Pei

AbstractDiscovered nearly 70 years ago, the allosteric regulation of Escherichia coli aspartate transcarbamoylase (ATCase) is discussed in every biochemistry textbook. ATCase catalyzes the first step in pyrimidine biosynthesis. Despite extensive research, the mechanism by which this enzyme is regulated by pyrimidine and purine nucleotides has remained elusive. Here, we present a detailed analysis of E.