Nicolas Mouquet

Jul 17, 2024 | science.org | Mahim Lele |Nicolas Mouquet |Dan Wu |Rafal Zdanowicz

AbstractThe pyruvate dehydrogenase complex (PDHc) is a key megaenzyme linking glycolysis with the citric acid cycle. In mammalian PDHc, dihydrolipoamide acetyltransferase (E2) and the dihydrolipoamide dehydrogenase-binding protein (E3BP) form a 60-subunit core that associates with the peripheral subunits pyruvate dehydrogenase (E1) and dihydrolipoamide dehydrogenase (E3). The structure and stoichiometry of the fully assembled, mammalian PDHc or its core remained elusive.

Jul 17, 2024 | science.org | Mahim Lele |Nicolas Mouquet |Dan Wu |Dong-Liang Huang

AbstractChemical ligation of peptides is increasingly used to generate proteins not readily accessible by recombinant approaches. However, a robust method to ligate “difficult” peptides remains to be developed. Here, we report an enhanced native chemical ligation strategy mediated by peptide conjugation in trifluoroacetic acid (TFA).

Jul 17, 2024 | science.org | Mahim Lele |Nicolas Mouquet |Dan Wu |Xiaomin Jiang

AbstractThe low success rate of cancer nanomedicines has raised debate on the role of the enhanced permeability and retention (EPR) effect on tumor deposition of nanotherapeutics. Here, we report a bifunctional nanoscale coordination polymer (NCP), oxaliplatin (OX)/2′,3′-cyclic guanosine monophosphate–adenosine monophosphate (GA), to overcome the EPR limitation through stimulator of interferon genes (STING) activation and enhance chemotherapeutic and STING agonist delivery for tumor eradication.

Jul 17, 2024 | science.org | Mahim Lele |Nicolas Mouquet |Dan Wu |Manish Kumar

AbstractDiatoms are major players in the global carbon cycle, and their metabolism is affected by ocean conditions. Understanding the impact of changing inorganic nutrients in the oceans on diatoms is crucial, given the changes in global carbon dioxide levels. Here, we present a genome-scale metabolic model (iMK1961) for Cylindrotheca closterium, an in silico resource to understand uncharacterized metabolic functions in this ubiquitous diatom.

Jul 17, 2024 | science.org | Mahim Lele |Nicolas Mouquet |Dan Wu |Yun Gao

AbstractPerovskite light-emitting diodes (PeLEDs) provide excellent opportunities for low-cost, color-saturated, and large-area displays. However, the performance of blue PeLEDs lags far behind that of their green and red counterparts. Here, we show that the external quantum efficiencies (EQEs) of blue PeLEDs scale linearly with the photoluminescence quantum yields (PL QYs) of CsPb(BrxCl1−x)3 nanocrystals emitting at 460 to 480 nm.