Oliver Kieran Castell

May 31, 2024 | nature.com | Pantelitsa Dimitriou |Jin Billy Li |William Jamieson |Johannes Schneider |Oliver Kieran Castell

AbstractDroplet Interface Bilayers (DIBs) constitute a commonly used model of artificial membranes for synthetic biology research applications. However, their practical use is often limited by their requirement to be surrounded by oil. Here we demonstrate in-situ bilayer manipulation of submillimeter, hydrogel-encapsulated droplet interface bilayers (eDIBs). Monolithic, Cyclic Olefin Copolymer/Nylon 3D-printed microfluidic devices facilitated the eDIB formation through high-order emulsification.