Peter Yoon

Dec 4, 2024 | nature.com | Yekaterina Shulgina |Marena Trinidad |Conner Langeberg |Hunter Nisonoff |Seyone Chithrananda |Petr Skopintsev | +7 more

AbstractStructured RNA lies at the heart of many central biological processes, from gene expression to catalysis. RNA structure prediction is not yet possible due to a lack of high-quality reference data associated with organismal phenotypes that could inform RNA function. We present GARNET (Gtdb Acquired RNa with Environmental Temperatures), a new database for RNA structural and functional analysis anchored to the Genome Taxonomy Database (GTDB).

Jul 18, 2024 | science.org | Jacob H Wilde |Anurag Verma |Zhi Yi Lin |Peter Yoon

AbstractThe RNA-guided ribonuclease CRISPR-Cas13 enables adaptive immunity in bacteria and programmable RNA manipulation in heterologous systems. Cas13s share limited sequence similarity, hindering discovery of related or ancestral systems. To address this, we developed an automated structural-search pipeline to identify an ancestral clade of Cas13 (Cas13an), and further trace Cas13 origins to defense-associated ribonucleases.