Rachel L. Dannenberg

Oct 17, 2024 | biorxiv.org | Rachel L. Dannenberg |Helen Wieffering Washington |Shijun Gao |Joseph A Cardina

AbstractDuring replication, lagging strand lesions are initially encountered by high fidelity DNA polymerase (pol) holoenzymes comprised of pol δ and the PCNA sliding clamp. To proceed unhindered, pol δ holoenzymes must bypass lesions without stalling. This entails dNMP incorporation opposite the lesion (insertion) and the 5' template nucleotide (extension).

Nov 8, 2023 | biorxiv.org | Kara G. Pytko |Rachel L. Dannenberg |Kristin A. Eckert |Mark Hedglin

AbstractDifficult-to-Replicate Sequences (DiToRS) are natural impediments in the human genome that inhibit DNA replication under endogenous replication. Some of the most widely-studied DiToRS are A+T-rich, high flexibility regions, including long stretches of perfect [AT/TA] microsatellite repeats that have the potential to collapse into hairpin structures when in single-stranded DNA (ssDNA) form and are sites of recurrent structural variation and double-stranded DNA (dsDNA) breaks.