Rafael Vazquez-Duhalt

Sep 22, 2024 | onlinelibrary.wiley.com | Maria Rivera |Rafael Vazquez-Duhalt |Ernestina Castro-Longoria |Karla Juarez-Moreno

1 INTRODUCTION Cancer is one of the significant illnesses worldwide, and breast cancer has overtaken lung cancer as the most often diagnosed disease in women.[1] Chemotherapy is still one of the best strategies for cancer treatment; however, as a result of the use of high doses of anticancer drugs, the tumor sensitivity to oncological therapeutics decreases while broad multidrug resistance (MDR) cancer phenotypes are more often detected.

Sep 1, 2024 | chemistry-europe.onlinelibrary.wiley.com | Rafael Vazquez-Duhalt |Sergio Ojeda Santillán

We describe the design and catalytic activity of enzymatic nanoreactors based on copper oxide nanoparticles surface-functionalized with laccase (CuO-Lac) from Coriolopsis gallica. Transmission electron micrographs show complete laccase coverage on the nanoparticle surfaces. In addition, the catalytic rate (kcat) of the immobilized laccase is two-fold higher at an optimized pH, when compared with free laccase, while the affinity constant (KM) is not significantly affected.

Aug 31, 2023 | chemistry-europe.onlinelibrary.wiley.com | Rafael Vazquez-Duhalt |Kanchan Chauhan |Prakhar Sengar |Andrea Dorado Baeza

Highly stable and reusable magnetic crosslinked enzyme aggregates (m-CLEAS) of laccase are synthesized with simultaneous improved enzymatic activity. Magnetic copper ferrite nanoparticles (CFNPs) were synthesized by solvothermal procedure with an average size of ~8 nm. The nanometric m-CLEAS were formed by co-aggregation of enzyme with CFNPs and crosslinked using glutaraldehyde.

Jul 7, 2023 | onlinelibrary.wiley.com | Patricia Juárez |Ana Rodríguez-Hernández |Rafael Vazquez-Duhalt |Pedro Gama

RESEARCH ARTICLE This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as https://doi.org/10.1002/biot.202300199 Abstract Background : Breast cancer is the most common malignant tumor disease and the leading cause of female mortality.