Sergio Ojeda Santillán

Sep 1, 2024 | chemistry-europe.onlinelibrary.wiley.com | Rafael Vazquez-Duhalt |Sergio Ojeda Santillán

We describe the design and catalytic activity of enzymatic nanoreactors based on copper oxide nanoparticles surface-functionalized with laccase (CuO-Lac) from Coriolopsis gallica. Transmission electron micrographs show complete laccase coverage on the nanoparticle surfaces. In addition, the catalytic rate (kcat) of the immobilized laccase is two-fold higher at an optimized pH, when compared with free laccase, while the affinity constant (KM) is not significantly affected.