Renata Espejel Moreno

Jan 18, 2025 | biorxiv.org | Luis Aguilar Yuste |Fernando Rojo |Renata Espejel Moreno |Elena Pares-Guillen

AbstractPseudomonas putida KT2440 contains seven copies of an insertion sequence (IS) belonging to the IS110 family, designated ISPpu9, which are inserted into repetitive extragenic palindromic sequences. Five of these copies include a small RNA, ssr9, located downstream of the transposase gene. Additionally, three separate copies of ssr9 are also present. When transferred to a different P. putida strain, ISPpu9 inserted at a specific target site highly similar to that of the KT2440 strain.

Jan 16, 2024 | ami-journals.onlinelibrary.wiley.com | Renata Espejel Moreno |CSIC Madrid Spain |Fernando Rojo

INTRODUCTION The utility of bacteria in biotechnology is indisputable. They can produce relevant compounds via biotransformation and fermentation procedures or reduce the impact of toxic waste. In some instances, specific bacteria can be employed without altering their genome, while in other scenarios specific genes must be inactivated, or novel ones introduced.