Renbo Mao

Apr 30, 2024 | elifesciences.org | Renbo Mao |Jianjun Yu |Bowen Deng |Xihuimin Dai

Request a detailed protocol For all imaging without staining, adult flies were anesthetized on ice and dissected in cold phosphate buffered saline (PBS). Brains or ventral nerve codes (VNCs) were fixed in 2% paraformaldehyde (weight/volume) for 30 min, washed with washing buffer (PBS with 1% Triton X-100, v/v, 3% NaCl, g/ml) for 7 min three times, and mounted in Focusclear (Cell Explorer Labs, FC-101).

Jan 17, 2024 | biorxiv.org | Renbo Mao |Jianjun Yu |Bowen Deng |Xihuimin Dai

AbstractDissection of neural circuitry underlying behaviors is a central theme in neurobiology. We have previously proposed the concept of chemoconnectome (CCT) to cover the entire chemical transmission between neurons and target cells in an organize and created tools for studying it (CCTomics) by targeting all genes related to the CCT in Drosophila. Here we have created lines targeting the CCT in conditional manners after modifying GFP RNA interference, Flp-out and CRISPR/Cas9 technologies.

Sep 27, 2023 | biorxiv.org | Jianjun Yu |Bowen Deng |Renbo Mao |Xihuimin Dai

AbstractDissection of neural circuitry underlying behaviors is a central theme in neurobiology. Chemical transmission is the predominant model for signaling between neurons. Here we have created lines target all chemical transmission corresponding genes in Drosophila after modifying GFP RNA interference, Flp-out and CRISPR/Cas9 technologies.