Roman M. Doll

Aug 30, 2023 | science.org | Annie Chen |Chen Li |Zinan Wang |Roman M. Doll

Research ArticleGENETICS INTRODUCTIONThe manipulation of genes through the induction of precise mutations is a central tool to establish causal genotype-phenotype relationships. Over the last decade, clustered regularly interspaced short palindromic repeats–Cas (CRISPR-Cas) systems have emerged as the preferred system for targeted genome manipulation, mainly due to their high efficiency and ease of use.