Rose Ruiz Daniels

Oct 17, 2023 | biorxiv.org | Sarah J. Salisbury |Rose Ruiz Daniels |Sean Monaghan |James E. Bron

AbstractBackground:Salmonid species have followed markedly divergent evolutionary trajectories in their interactions with sea lice. While sea lice parasitism poses significant economic, environmental, and animal welfare challenges for Atlantic salmon (Salmo salar) aquaculture, coho salmon (Oncorhynchus kisutch) exhibit near-complete resistance to sea lice, achieved through a potent epithelial hyperplasia response leading to rapid louse detachment.

Sep 14, 2023 | paperity.org | Rose Ruiz Daniels |Richard Taylor |Ross Dobie |Sarah J. Salisbury

PLOS ONE, Sep 2023 The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie.

Sep 7, 2023 | journals.plos.org | Richard Taylor |Ross Dobie |Edinburgh Bioquarter |Rose Ruiz Daniels

Citation: Ruiz Daniels R, Taylor RS, Dobie R, Salisbury S, Furniss JJ, Clark E, et al. (2023) A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues. PLoS ONE 18(9): e0285020.