Ross Dobie

Sep 14, 2023 | paperity.org | Rose Ruiz Daniels |Richard Taylor |Ross Dobie |Sarah J. Salisbury

PLOS ONE, Sep 2023 The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie.

Sep 7, 2023 | journals.plos.org | Richard Taylor |Ross Dobie |Edinburgh Bioquarter |Rose Ruiz Daniels

Citation: Ruiz Daniels R, Taylor RS, Dobie R, Salisbury S, Furniss JJ, Clark E, et al. (2023) A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues. PLoS ONE 18(9): e0285020.