Rudbeck Laboratory

Feb 25, 2024 | actaneurocomms.biomedcentral.com | Rudbeck Laboratory

Human induced pluripotent stem cell (iPSC)-derived neuroepithelial stem (NES) cells (iPSCs, Cntrl9 II cell line) were differentiated into astrocytes following a well-established 28-day protocol with some minor changes [18, 37]. First, NES cells were cultured in cell culture flasks (Sarstedt) pre-coated with 100 µg/ml poly-L-ornithine (Sigma, P3655) and 50 µg/ml laminin (Sigma, L2020).

Jan 23, 2024 | academic.oup.com | Rudbeck Laboratory

During the last three decades, the level of DNA damage in individual cells has been evaluated using single-cell gel electrophoresis under neutral (1,2) or alkaline conditions (3,4). The latter version, also known as the comet assay, has been used when evaluating the potential genotoxicity of chemicals, to study DNA-repair, and for biomonitoring purposes (5–9).

Jun 17, 2023 | actaneurocomms.biomedcentral.com | Rudbeck Laboratory

Culture of human iPSC-derived astrocytesHuman astrocytes were differentiated from neuroepithelial-like stem (NES) cells, produced from human induced pluripotent stem cells (iPSCs, Cntrl9 II cell line) [24].

May 19, 2023 | frontiersin.org | Shaanxi Normal |Rudbeck Laboratory |David Eisenstat

The blood-brain barrier (BBB) is a specialization of brain endothelial cells (ECs) that hinders the effective delivery of drugs into the brain by establishing EC junctions, sealing EC-EC contacts, pumping drugs out by active transport, and suppressing transcytosis (1, 2). In brain tumors, blood vessels are abnormal with a heterogeneous and partially intact BBB, known as the blood-tumor barrier (BTB) (1, 3).