Sam Kovaka

Mar 11, 2024 | biorxiv.org | Sam Kovaka |Paul W. Hook |Katharine Jenike |Vikram S. Shivakumar

AbstractNanopore signal analysis enables detection of nucleotide modifications from native DNA and RNA sequencing, providing both accurate genetic/transcriptomic and epigenetic information without additional library preparation. Presently, only a limited set of modifications can be directly basecalled (e.g. 5-methylcytosine), while most others require exploratory methods that often begin with alignment of nanopore signal to a nucleotide reference.