Samuel Itskanov

Jan 9, 2024 | biorxiv.org | Kevin Wu |Samuel Itskanov |Diane Lynch |Yuanyuan Chen

AbstractDoa10 (MARCH6 in metazoans) is a large polytopic membrane-embedded E3 ubiquitin ligase in the endoplasmic reticulum (ER) that plays an important role in quality control of cytosolic and ER proteins. Although Doa10 is highly conserved across eukaryotes, it is not understood how Doa10 recognizes its substrates. Here, we defined the substrate recognition mechanism of Doa10 by structural and functional analyses on Saccharomyces cerevisiae Doa10 and its well-defined degron Deg1.

May 11, 2023 | nature.com | Samuel Itskanov |Laurie Wang |Nicolas Blanchard |Wei Shi |Martin Spiess |Rumi Sherriff | +1 more

Extended Data Fig. 1 Cryo-EM analysis of the yeast and human Sec complexes. a, A schematic of the single-particle cryo-EM analysis of the yeast Sec (ScSec) complex incubated with cotransin CP2. Note that the particles were sorted into two 3D classes, with and without Sec62, due to partial occupancy of Sec62. b, 3D reconstructions of the ScSec complex with and without ScSec62 (shown in yellow). No cotransin-like density was observed in either class.