Samuel Okyem

Nov 18, 2024 | analyticalsciencejournals.onlinelibrary.wiley.com | Jonathan V. Sweedler |Samuel Okyem

1 Introduction After performing a high-quality LC-MS based bottom-up proteomics experiment, an observant individual may notice a few cases where the same peptide is assigned to two distinct retention time peaks. If the retention times differ significantly, one can ask how this is possible? What properties caused the two peptides to separate in an LC run but to have the same assigned sequence?