Jonathan V. Sweedler

3 weeks ago | nature.com | Pei Su |Stanislav S. Rubakhin |Michael Caldwell |Jonathan V. Sweedler

AbstractWe perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.

Jan 14, 2025 | dx.doi.org | Jonathan V. Sweedler

As I look forward to 2025, which marks the publication of Analytical Chemistry’s 97th volume, I find myself also reflecting on some recent successes. I am in my 19th year with Analytical Chemistry─14 as the Editor-in-Chief (EIC) and five as an Associate Editor (AE)─and the one constant over this period has been growth.

Jan 14, 2025 | pubs.acs.org | Jonathan V. Sweedler

As I look forward to 2025, which marks the publication of Analytical Chemistry’s 97th volume, I find myself also reflecting on some recent successes. I am in my 19th year with Analytical Chemistry─14 as the Editor-in-Chief (EIC) and five as an Associate Editor (AE)─and the one constant over this period has been growth.

Nov 18, 2024 | analyticalsciencejournals.onlinelibrary.wiley.com | Jonathan V. Sweedler |Samuel Okyem

1 Introduction After performing a high-quality LC-MS based bottom-up proteomics experiment, an observant individual may notice a few cases where the same peptide is assigned to two distinct retention time peaks. If the retention times differ significantly, one can ask how this is possible? What properties caused the two peptides to separate in an LC run but to have the same assigned sequence?

Feb 16, 2024 | nature.com | Yuxuan Xie |Daniel Castro |Stanislav S. Rubakhin |Jonathan V. Sweedler

AbstractSpatial omics technologies can reveal the molecular intricacy of the brain. While mass spectrometry imaging (MSI) provides spatial localization of compounds, comprehensive biochemical profiling at a brain-wide scale in three dimensions by MSI with single-cell resolution has not been achieved. We demonstrate complementary brain-wide and single-cell biochemical mapping using MEISTER, an integrative experimental and computational mass spectrometry (MS) framework.