Sebastian Glatt

Nov 15, 2024 | nature.com | Liu Wang |Sebastian Glatt

AbstractRNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily determine their functions, it remains challenging to experimentally determine these 3D structures due to their conformational heterogeneity and intrinsic dynamics.

Oct 3, 2024 | nature.com | Sebastian Pintscher |Rafał Pietras |Bohun Mielecki |Mateusz Szwalec |Anna Wójcik-Augustyn |Paulina Indyka | +3 more

AbstractA multi-subunit enzyme, cytochrome b6f (cytb6f), provides the crucial link between photosystems I and II in the photosynthetic membranes of higher plants, transferring electrons between plastoquinone (PQ) and plastocyanin. The atomic structure of cytb6f is known, but its detailed catalytic mechanism remains elusive.

Aug 8, 2023 | nature.com | Lukasz Koziej |Igor Kaczmarczyk |Monika Gaik |Michal Rawski |Sebastian Glatt |Panagiotis Poulis

AbstractN6-methyladenosine (m6A) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. m6A in the mRNA coding regions inhibits translation elongation. Here, we show how m6A modulates decoding in the bacterial translation system using a combination of rapid kinetics, smFRET and single-particle cryo-EM.

Mar 27, 2023 | nature.com | Piotr Wilk |Artur Biela |Michał Rawski |Gert Bange |Sebastian Glatt

AbstractHypusination is a unique post-translational modification of the eukaryotic translation factor 5A (eIF5A) that is essential for overcoming ribosome stalling at polyproline sequence stretches. The initial step of hypusination, the formation of deoxyhypusine, is catalyzed by deoxyhypusine synthase (DHS), however, the molecular details of the DHS-mediated reaction remained elusive. Recently, patient-derived variants of DHS and eIF5A have been linked to rare neurodevelopmental disorders.

Mar 2, 2023 | nature.com | Sebastian Glatt

AbstractBackgroundNeurodevelopmental disorders (NDDs) are heterogeneous, debilitating conditions that include motor and cognitive disability and social deficits. The genetic factors underlying the complex phenotype of NDDs remain to be elucidated. Accumulating evidence suggest that the Elongator complex plays a role in NDDs, given that patient-derived mutations in its ELP2, ELP3, ELP4 and ELP6 subunits have been associated with these disorders.